Trying another breeding experiment

Last week we tried to setup another breeding experiment. It was unsuccessful, but we are hopeful now that our water chemistry has 'cycled' after break we will return to trying to get embryos.

We still have 7 larval stage fish

Zebrafish Catching Prey

FishNet for Zebrafish Anatomy

Here is a cool website (courtesy of Monash University) that allows you to look at cross-sections of zebrafish from various angles and at various stages of development. Check the box marked "annotations" and the image will include labels for various parts of the fish (e.g., "notochord," "yolk," and "myotome"). This is an easy and interactive way to familiarize yourself with zebrafish embryonic and larval structures.

Setting up a breeding experiment!

Zebra fish require an asynchronous breeding schedule (Lawrence, 2007 Aquaculture).

This week we set up a breeding experiment with fish from the TL line, and one with TL and AB line fish. These experiments did not result in any embryos which could be from the requirement of zebra fish mating that relies on pheromones and other signals (Lawrence, 2007 Aquaculture) or our issues with water chemistry.

Zebra Fish in the Classroom

This is a great link to resources for using Zebra Fish in the classroom.

ZFIC.ORG

Embryo Development

You can find several videos of embryo development on YouTube.  This one shows 24 hrs of development in  less than one minute.

We hope at a later date to post our own videos of our developing embryos!

Our First Embryos!

We crossed wild type fish from the TL line (courtesy of the Burdine Lab at Princeton University) and recovered 28 embryos which were moved to egg water on November 29

TL Line